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31.
Abstract The few existing nucleoside transport inhibitors are almost equipotent on the transporter in washed human erythrocytes. Large differences exist however in tightness of binding and activity in the presence of human plasma, as well as in ex vivo inhibition and duration of action when given intravenously or orally. Examples are presented of the marked effect of nucleoside transport inhibition on adenosine accumulation in ischemic myocardium. That may explain the remarkable cardioprotection in several models when a nucleoside transport inhibitor with an appropriate pharmacokinetic profile (such as R 75 231) is applied. 相似文献
32.
A. Van Aerschot P. Herdewijn G. Janssen H. Vanderhaeghe 《Nucleosides, nucleotides & nucleic acids》2013,32(4):519-536
Abstract The reaction of O-protected inosine with p-nitrophenyl ethanol under Mitsunobu conditions yields a mixture of the 1- and 0° -alkylated derivatives. 2′-Deoxyinosine protected on 06 -, can be synthesized fairly easy from deoxyguanosine with a Mitsunobu reaction followed by reductive deamination. 相似文献
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J J Schalken J J Janssen W J de Grip R K Hawkins S Sanyal 《Biochimica et biophysica acta》1985,839(1):122-126
In 020/A mice, homozygous for the retinal degeneration slow (rds) gene, the photoreceptor cells fail to develop outer segments, and in the absorption spectra of retinal extracts the rhodopsin peak is lacking. Application of an enzyme-linked immunoassay using antisera against bovine opsin shows, however, that opsin is present in the homozygous mutant retina (0.010 nmol/eye) at 3% of the level of the normal retina (0.38 nmol/eye) of Balb/c mice. In the retina of heterozygous mice the opsin level (0.19 nmol/eye) is about half of the normal. Detection of opsin in the rds mutant retina demonstrates the functional basis for the reported electroretinographic response and light-mediated reduction in cyclic nucleotide levels in this mutant. 相似文献
35.
Pascal Bonnarme Serge Moukha Patrick Moreau Eric Record Laurence Lesage Claude Cassagne Marcel Asther 《FEMS microbiology letters》1994,120(1-2):155-161
Abstract Mycelia from the basidiomycete Phanerochaete chrysosporium , producing lignin and manganese peroxidases, were homogenized and fractionated on a sucrose gradient. The main subcellular fungal membrane fractions were successfully separated. Lipid composition analyses of the isolated membranes as well as associated marker enzymes distribution gave evidence to similarities with membranes originating from plants. Lignin and manganese peroxidases were investigated by immunodetection in subcellular fractions. Our results show that lignin and manganese peroxidases are mainly associated with Golgi apparatus vesicles and, to a lesser extent, with endoplasmic reticulum and light density vesicles, but not with plasma membranes. 相似文献
36.
Arild Husby Loeske E.B. Kruuk Marcel E. Visser 《Proceedings. Biological sciences / The Royal Society》2009,276(1663):1845-1854
For multiple-brooded species, the number of reproductive events per year is a major determinant of an individual''s fitness. Where multiple brooding is facultative, its occurrence is likely to change with environmental conditions, and, as a consequence, the current rates of environmental change could have substantial impacts on breeding patterns. Here we examine temporal population-level trends in the proportion of female great tits (Parus major) producing two clutches per year (‘double brooding’) in four long-term study populations in The Netherlands, and show that the proportion of females that double brood has declined in all populations, with the strongest decline taking place in the last 30 years of the study. For one of the populations, for which we have data on caterpillar abundance, we show that the probability that a female produces a second clutch was related to the timing of her first clutch relative to the peak in caterpillar abundance, and that the probability of double brooding declined over the study period. We further show that the number of recruits from the second clutch decreased significantly over the period 1973–2004 in all populations. Our results indicate that adjustment to changing climatic conditions may involve shifts in life-history traits other than simply the timing of breeding. 相似文献
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38.
Abstract The conversion of the benzoylated cAMP (2) to the diastereomeric mixture of the anilidates (3) was improved. Replacing the triphenylphosphine/carbon tetrachloride mixture by oxalyl chloride in the presence of a catalytic amount of DMF followed by the addition of aniline not only increased the yield from 27 to 96% but rendered much easier the separation of the diastereoisomers (3), which were formed in approximately equal amounts. This greatly improved the accessability of Rp and Sp-cAMPS (1). 相似文献
39.
Lennard van der Woude Markus Piotrowski Gruson Klaasse Judith K. Paulus Daniel Krahn Sabrina Ninck Farnusch Kaschani Markus Kaiser Ondřej Novák Karin Ljung Suzanne Bulder Marcel van Verk Basten L. Snoek Martijn Fiers Nathaniel I. Martin Renier A. L. van der Hoorn Stéphanie Robert Sjef Smeekens Martijn van Zanten 《The Plant journal : for cell and molecular biology》2021,106(6):1523-1540
Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf-cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4-2–deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound ‘Heatin’, containing 1-iminomethyl-2-naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin-mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1-subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin-mediated hypocotyl elongation requires the NITRILASE1-subfamily members, NIT1 and NIT2. Heatin inhibits NIT1-subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1-subfamily substrate indole-3-acetonitrile in vivo. However, levels of the NIT1-subfamily product, bioactive auxin (indole-3-acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1-subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole-3-acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology. 相似文献